胰蛋白酶-EDTA 溶液

Technical information for working with human coronary artery smooth muscle cells including thawing, subculturing and cryopreservation

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.