T3924
胰蛋白酶-EDTA 溶液
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
别名:
Cell dissociation enzyme
产品名称
胰蛋白酶-EDTA 溶液, 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
biological source
Porcine
sterility
sterile; sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-9.0
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
适合用于制备测序用单细胞悬液。
Biochem/physiol Actions
丝氨酸蛋白酶抑制剂（包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等）会抑制胰蛋白酶。
Disclaimer
Other Notes
Preparation Note
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
商品
Technical information for working with human coronary artery smooth muscle cells including thawing, subculturing and cryopreservation
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
实验方案
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
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