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T4415

Tris-硼酸-EDTA缓冲溶液

BioReagent, suitable for electrophoresis, 10× concentrate

别名:

TBE 缓冲液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
329826793
UNSPSC Code:
41105319
MDL number:
MFCD00236358
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Quality Level

200

product line

BioReagent

form

solution

impurities

DNase and RNase, none detected

suitability

suitable for electrophoresis

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

General description

TBE（Tris/硼酸/EDTA)缓冲液通常用于核酸电泳。该溶液在弱碱性条件下有效，能够保持DNA去质子化，水溶性，免于降解。该浓缩溶液能够方便地在使用前稀释到1x或0.5x（分子生物级别水）。

Application

稀释到工作浓度后，可用于凝胶电泳。
Tris-硼酸-EDTA缓冲液用于脉冲电场凝胶电泳。
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

Packaging

4L、10L 和 20L 容量用带龙头分液器包装。

Preparation Note

用 18 兆欧水制备
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

Other Notes

TBE 缓冲剂会逐渐沉淀。通常而言，沉淀不会影响性能。

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000504735
0000504735
0000420948
0000420948
0000463005

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实验方案

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

查看所有协议
M Wu et al.
BioTechniques, 24(4), 676-678 (1998-06-13)
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose
L Orbán et al.
Electrophoresis, 12(4), 233-240 (1991-04-01)
DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. Polyacrylamide gels at pH 8.9, 2 degrees
Characterization of a Clavibacter michiganensis subsp. michiganensis population in Israel.
Kleitman, F., Barash, I., Burger, A., Iraki, N.,
European Journal of Plant Pathology, 121, 463-475 (2008)
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全球贸易项目编号

货号GTIN
T4415-10L04061838260239
T4415-1L04061838260246
T4415-20L04061838101280
T4415-4L04061838260253