T4444
3, 3′,5 ,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, aqueous solution
别名:
TMB
产品名称
3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA, ready to use solution
质量水平
表单
aqueous solution
运输
wet ice
储存温度
2-8°C
SMILES字符串
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
一般描述
本品为包含3,3′,5,5′-四甲基联苯胺（TMB）的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。
应用
3,3′,5,5′-四甲基联苯胺（TMB）是适用于ELISA程序的过氧化物酶底物。底物产生可溶的浅蓝色最终产物，并且可以在370或655nm下通过分光光度计读取。可以用2M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。
的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：
- 通过转染子测定合成的透明质酸和HAS2（透明质酸合成酶）表达
- 测定结合的α-溶血素毒素的水平
- 结合IgG成像
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Ruud van den Bos et al.
Behavioural pharmacology, 30(2 and 3-Spec Issue), 260-271 (2019-02-07)
Maternal stress and early life stress affect development. Zebrafish (Danio rerio) are ideally suited to study this, as embryos develop externally into free-feeding larvae. The objective of this study was therefore to assess the effects of increased levels of cortisol
The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination
Karousou E, et al.
The Journal of Biological Chemistry, 285(31), 23647-23654 (2010)
E Brandon Strong et al.
Scientific reports, 9(1), 7-7 (2019-01-11)
Microfluidic paper-based analytical devices (microPADs) are emerging as cost-effective and portable platforms for point-of-care assays. A fundamental limitation of microPAD fabrication is the imprecise nature of most methods for patterning paper. The present work demonstrates that paper patterned via wax
Runhong Zhou et al.
Immunity, 53(4), 864-877 (2020-08-14)
The SARS-CoV-2 pandemic has resulted in millions of infections, yet the role of host immune responses in early COVID-19 pathogenesis remains unclear. By investigating 17 acute and 24 convalescent patients, we found that acute SARS-CoV-2 infection resulted in broad immune
E6 and E7 Antibody Levels Are Potential Biomarkers of Recurrence in Patients with Advanced-Stage Human Papillomavirus-Positive Oropharyngeal Squamous Cell Carcinoma.
Matthew E Spector et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 23(11), 2723-2729 (2016-11-23)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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