T5398
转谷氨酰胺酶 来源于天竺鼠肝脏
lyophilized powder, ≥1.5 units/mg protein
别名:
蛋白质-谷氨酰胺-γ-谷氨酰转移酶, 蛋白质-谷氨酰胺:胺γ-谷氨酰转移酶
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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1.5 units/mg protein
产品名称
转谷氨酰胺酶 来源于天竺鼠肝脏, lyophilized powder, ≥1.5 units/mg protein
form
lyophilized powder
specific activity
≥1.5 units/mg protein
mol wt
76.6 kDa
composition
Protein, ≥80% modified Warburg-Christian
solubility
H2O: soluble 1.0 mg/mL, clear
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
Sigma的该产品已用于证明组织转谷氨酰胺酶（tTG）选择性地使谷蛋白肽脱酰胺，这导致强烈增强的T细胞刺激活性。其还可用于评估对 A-麦醇溶蛋白肽的免疫应答。此外，它已用于证明 tTG 选择性地修饰在乳糜泻中由肠源性 T细胞识别的麦醇溶蛋白肽。
转谷氨酰胺酶已用于一项可改善可量化分析研究，以充分表征转谷氨酰胺酶在亨廷顿氏′s病和阿尔茨海默′s病等疾病中的作用。转谷氨酰胺酶还用于研究转谷氨酰胺酶活性的非放射性斑点印迹试验。
Biochem/physiol Actions
来自豚鼠肝脏的转谷氨酰胺酶由691个氨基酸残基的单一多肽链组成。它有六个潜在的糖基化位点（Asn-X-Ser或Asn-X-Thr），但它没有糖基化。分子量约为76.6kDa。它是钙依赖性的，有几个钙结合位点。在钙存在下，碘乙酰胺和N-乙基马来酰亚胺抑制该酶的活性。它催化小分子量胺类结合到蛋白质的γ-谷氨酰胺位点。在不存在小分子量胺类的情况下，它催化蛋白质的交联，导致 γ-谷氨酰基-ε-赖氨酸侧链肽的形成。肝转谷氨酰胺酶是非酶原的酶。
Other Notes
一个单元，在pH6.0，37℃下，每分钟将催化Nα-Z-Gln-Gly和羟胺形成1.0 μmole的异羟肟酸酯。（L-谷氨酸γ-单羟肟酸酯为参考标准。）
Physical form
含 Tris 和二硫赤藓醇的冻干粉
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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Mechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity.
J E Folk et al.
The Journal of biological chemistry, 241(23), 5518-5525 (1966-12-10)
L Lorand et al.
Molecular and cellular biochemistry, 58(1-2), 9-35 (1984-01-01)
This paper is intended as a background to the topic of transglutaminases, while focusing on current ideas regarding the biological roles of these enzymes. Specifically, the following topics are discussed: geometry of forming gamma-glutamyl-epsilon-lysine cross-linked structures; energetic considerations; the gamma-glutamyl-epsilon-lysine
R P Anderson et al.
Nature medicine, 6(3), 337-342 (2000-03-04)
Celiac disease (CD) is an increasingly diagnosed enteropathy (prevalence, 1:200-1:300) that is induced by dietary exposure to wheat gliadins (as well as related proteins in rye and barley) and is strongly associated with HLA-DQ2 (alpha1*0501, beta1*0201), which is present in
M P M Adriaanse et al.
Alimentary pharmacology & therapeutics, 37(4), 482-490 (2013-01-08)
Enterocyte damage is the hallmark of coeliac disease (CD) resulting in malabsorption. Little is known about the recovery of enterocyte damage and its clinical consequences. Serum intestinal fatty acid binding protein (I-FABP) is a sensitive marker to study enterocyte damage.
相关内容
Product Information Sheet
QC Methods
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