Merck
CN

T5525

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺

tablet, 1 mg substrate per tablet

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别名:
BM蓝, Sure Blue TMB, TMB, TMB印迹Plus, TMB底物
线性分子式:
[-C6H2(CH3)2-4-NH2]2
CAS号:
分子量:
240.34
Beilstein:
2808541
MDL编号:
PubChem化学物质编号:
NACRES:
NA.83

质量水平

形式

tablet

mp

168-171 °C (lit.)

储存温度

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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此商品
T288587748860336
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

168-171 °C (lit.)

mp

168-171 °C (lit.)

mp

166-171 °C, 168-171 °C (lit.)

mp

168-171 °C (lit.)

应用

3,3′,5,5′-四甲基联苯胺已用作竞争法和间接法酶联免疫吸附分析(ELISA)的底物。
3,3′,5,5′-四甲基联苯胺(TMB)是一种二氨基联苯胺的非致癌性取代物(Ames测试阴性)适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物,淡蓝色,分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H2SO4终止(产生黄色),在450nm处可读。一种高敏感,特异性试剂用于检测血液,血红蛋白实验,过氧化物酶实验。

象形图

Exclamation markHealth hazard

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Lactobacillus casei LcY decreases milk protein immunoreactivity of fermented buttermilk but also contains IgE-reactive proteins
Wroblewska B, et al.
Food Research International, 83, 95-101 (2016)
Effect of acylation and enzymatic modification on pea proteins allergenicity
Szymkiewicz, A and Jedrychowski, L
Polish Journal of Food and Nutrition Sciences, 58(3) (2008)
Immunoreactive properties of peptide fractions of cow whey milk proteins after enzymatic hydrolysis
Wroblewska B, et al.
Polish Journal of Food and Nutrition Sciences, 39(8), 839-850 (2004)
Simon Z He et al.
Leukemia & lymphoma, 56(5), 1406-1415 (2014-09-25)
Acute myeloid leukemia (AML) blasts express high levels of interlekin-3 (IL-3) receptor-α (CD123). CSL360 is a recombinant, chimeric immunoglobulin G1 (IgG1), anti-CD123 monoclonal antibody (MoAb) that neutralizes IL-3 and demonstrates anti-leukemic activity in vitro. This phase 1 study assessed safety
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given

商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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