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Merck
CN

T5569

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

别名:

TMB substrate for ELISA (Super Slow)

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UNSPSC Code:
12352204
NACRES:
NA.83
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产品名称

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate

form

liquid

shipped in

wet ice

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

相关内容

Instructions


Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis
Buatois V, et al.
Journal of Immunology, 185(4), 2544-2554 (2010)
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z, 39-40 (2017)
Shiran Su et al.
PloS one, 17(10), e0276107-e0276107 (2022-10-19)
The blood-brain barrier (BBB) presents a major obstacle in developing specific diagnostic imaging agents for many neurological disorders. In this study we aimed to generate single domain anti-mouse transferrin receptor antibodies (anti-mTfR VHHs) to mediate BBB transcytosis as components of



全球贸易项目编号

货号GTIN
T5569-4X100ML04061837347986
T5569-100ML04061837347962