产品名称
磷酸丙糖异构酶 来源于兔肌肉, Type X, lyophilized powder, ≥3,500 units/mg protein
type
Type X
form
lyophilized powder
specific activity
≥3,500 units/mg protein
mol wt
calculated mol wt 53.2 kDa
composition
Protein, 60-90% biuret
foreign activity
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
storage temp.
−20°C
Quality Level
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Application
来自兔肌肉的磷酸丙糖异构酶可与 p-羟基苯甲酸汞(PHMB)形成复合物,使蛋白质变性,从而进行疏水相互作用色谱,并在线与化学气相色谱-荧光光谱法(HIC−CVGAFS)耦合。
磷酸丙糖异构酶已被用于研究高脂饮食喂养的超重大鼠中心脏能量代谢途径的变化。 磷酸丙糖异构酶已被用于研究在1-溴丙烷暴露后F344大鼠海马体中羰基化蛋白的蛋白质组学鉴定。
Biochem/physiol Actions
磷酸丙糖异构酶(TPI)负责磷酸三糖异构体、磷酸二羟基丙酮和3-磷酸甘油醛的催化。 TPI缺乏与糖酵解酶病有关。 TPI的缺乏还与慢性溶血性贫血、细菌暴露感染、心肌病和神经肌肉疾病有关。
General description
磷酸丙糖异构酶(TPI)的基因位于人类12p13染色体上。它在所有组织中都有表达。其是由两个27 kDa亚基组成的同型二聚体,具有高度稳定性,由248个氨基酸组成。TPI在其二聚状态下具有催化活性。
Other Notes
一个酶活性单位是指在pH 7.6、温度25 °C条件下,每分钟将1.0 μmole的D-甘油醛-3-磷酸转化为二羟丙酮磷酸所需的酶量。
Packaging
包装尺寸基于蛋白质含量
Physical form
不含硫酸盐,含EDTA和硼酸盐缓冲盐
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Red blood cell enzymopathies
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Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet
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Hydrophobic interaction chromatography coupled with atomic fluorescence spectrometric detection: Effect of the denaturation on the determination of thiolic proteins
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Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1792(12), 1168-1174 (2009)
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Toxicology and applied pharmacology, 263(1), 44-52 (2012-06-12)
1-Bromopropane (1-BP) is neurotoxic in both experimental animals and humans. Previous proteomic analysis of rat hippocampus implicated alteration of protein expression in oxidative stress, suggesting that oxidative stress plays a role in 1-BP-induced neurotoxicity. To understand this role at the
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