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T6414

胰蛋白酶抑制剂来源于大豆

Name: 胰蛋白酶抑制剂 来源于<I>大豆</I>
Brand: SIGMA

solution, sterile-filtered, suitable for, suitable for cell culture

别名:

SBTI

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关于此项目

化学文摘社编号:

9035-81-8

UNSPSC Code:

12352204

NACRES:

NA.75

MDL number:

MFCD00082103

Biological source:

Glycine max (soybean)

Concentration:

10 mg/mL

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biological source

Glycine max (soybean)

Quality Level

100

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

20,100 Da

concentration

10 mg/mL

technique(s)

cell culture | mammalian: suitable

solubility

balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble

suitability

suitable for

UniProt accession no.

P01070

shipped in

dry ice

storage temp.

−20°C

Gene Information

Glycine max (soybean) ... KTI3(547831)

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Application

针对内皮细胞培养物传代进行了优化。
来源于大豆的胰蛋白酶抑制剂用于以下应用：

分散的腺泡的过滤和接种
磷酸化 tau 和免疫印迹分析的相对定量
VIL-4 和 IL-4 cDNA 的亚克隆化和转化，以及蛋白质表达和纯化（来源于大豆的胰蛋白酶抑制剂作为阳性对照）。

Biochem/physiol Actions

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

Preparation Note

在 Dulbecco′s 磷酸盐缓冲盐水中制备，不含钙和镁。

胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。

在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

Other Notes

一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A₂₅₃所需的酶量。

含有色谱纯化的大豆胰蛋白酶抑制剂和补铁小牛血清。

查看更多关于胰蛋白酶抑制剂的信息。

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000534608

0000479899

0000473411

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全球贸易项目编号

货号	GTIN
T6414-100ML	04061837371479
T6414-6X100ML	04061837371486

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