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Merck
CN

T6414

胰蛋白酶抑制剂 来源于大豆

solution, sterile-filtered, suitable for, suitable for cell culture

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SBTI

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化学文摘社编号:
9035-81-8
UNSPSC Code:
12352204
NACRES:
NA.75
MDL number:
MFCD00082103

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产品名称

胰蛋白酶抑制剂 来源于大豆, solution, sterile-filtered, suitable for, suitable for cell culture

biological source

Glycine max (soybean)

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

20,100 Da

concentration

10 mg/mL

technique(s)

cell culture | mammalian: suitable

solubility

balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble

suitability

suitable for

UniProt accession no.

P01070

shipped in

dry ice

storage temp.

−20°C

Quality Level

100

Gene Information

Glycine max (soybean) ... KTI3(547831)

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Application

针对内皮细胞培养物传代进行了优化。
来源于大豆的胰蛋白酶抑制剂用于以下应用：
  • 分散的腺泡的过滤和接种
  • 磷酸化 tau 和免疫印迹分析的相对定量
  • VIL-4 和 IL-4 cDNA 的亚克隆化和转化，以及蛋白质表达和纯化（来源于大豆的胰蛋白酶抑制剂作为阳性对照）。

Biochem/physiol Actions

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

Other Notes

一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A253所需的酶量。
含有色谱纯化的大豆胰蛋白酶抑制剂和补铁小牛血清。
查看更多关于 胰蛋白酶抑制剂的信息。

Preparation Note

在 Dulbecco′s 磷酸盐缓冲盐水中制备，不含钙和镁。
胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。

在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000479899
0000479899
0000473411
0000473411
0000429062

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