Porcine pancreas
BioReagent
1 mM HCl: soluble 1 mg/mL, clear, colorless
wet ice
2-8°C
Danger
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Respiratory system
11 - Combustible Solids
WGK 1
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。
为方便起见,与您过往购买产品相关的文件已保存在文档库中。
如何查找产品货号
在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ (Lot或Batch)字后面找到。
如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。
如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。
如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。
部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
This product is soluble in 1mM HCL.
Each vial content 20 ug of the product.
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.
As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
Ask a Scientist here.
化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
See how pretreatment with a mucin-selective protease, Mucinase StcE, for tryptic-digest sample preparation of mucins may increase the number of glycopeptides and glycoforms identified from your samples.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯(BAEE)作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定(A253,光程= 1cm)方案:
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