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Merck
CN

T6884

Thrombin 来源于人类血浆

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

别名:

IIa因子

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关于此项目

化学文摘社编号:
9002-04-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
MFCD00082072
EC 号:
3.4.21.5 (BRENDA, IUBMB)

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产品名称

Thrombin 来源于人类血浆, lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

biological source

human plasma

form

lyophilized powder

specific activity

≥2,000 NIH units/mg protein (E1%/280, 18.3)

mol wt

37.4 kDa

impurities

HIV and HBsAg, source material tested negative

UniProt accession no.

P00734

storage temp.

−20°C

Quality Level

200

Gene Information

human ... F2(2147)

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Analysis Note

NIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

Application

人血浆凝血酶已用于：
  • 的制备纤维蛋白凝胶，以评估人骨髓基质细胞(hBMSC)形态，
  • 血小板活化，
  • 评估内皮细胞（EC）单层完整性

凝血酶被用于重组融合蛋白的位点特异性切割，这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。

Biochem/physiol Actions

凝血酶的主要功能是将纤维蛋白原裂解为纤维蛋白，帮助稳定凝块形成。凝血酶原基因的广泛突变造成自身缺陷，导致凝血异常，如凝血酶原异常和凝血酶原低下。高水平的凝血酶可在多巴胺能神经元中引起神经毒性，并导致帕金森病恶化。通过调整凝血酶水平，可调节多发性硬化症的凝血途径。冠心病（CAD）患者的凝血酶水平会升高。凝血酶在大脑神经原纤维缠结中积累可能有助于tau蛋白聚集，形成阿尔茨海默氏病病理。
凝血酶被用于重组融合蛋白的位点特异性切割，这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶在一项研究中被用于研究蛋白C通路在肠屏障功能中的作用。
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。

Disclaimer

供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

General description

凝血酶是止血过程中的最终凝血蛋白酶，可同时促进促凝血和抗凝血作用。
凝血酶由肝脏中的非活性凝血酶原蛋白水解产生。它是具有四个结构域的糖蛋白：包含Gal域的10 γ羧化谷氨酸，两个kringle域和胰蛋白酶样丝氨酸蛋白酶结构域。凝血酶原基因位于人类染色体11p11.2上。

Other Notes

查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
直接与NIH凝血酶参考标准比较而得到NIH单位表示的活性

Physical form

由生理盐水柠檬酸钠缓冲液，pH 6.5，制得冻干粉

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000475769
0000475769
0000475768
0000475768
0000441438

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can a stock solution of Product T6884, Thrombin, be stored frozen?

    A suggested stock solution is 100 units/ml. The solution should contain 0.1% BSA for stability and may be stable for about one week at 0-5°C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at -20°C or below.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Thrombin generation correlates with disease duration in multiple sclerosis (MS): Novel insights into the MS-associated prothrombotic state
Parsons MEM, et al.
Multiple Sclerosis Journal, 3(4) (2017)
Silvia D'Alessio et al.
Annals of the New York Academy of Sciences, 1258, 78-85 (2012-06-27)
The protein C (PC) pathway is a well-characterized anticoagulant system. Produced mainly by the liver as a zymogen, PC is activated on the vascular endothelial cell surface by thrombin-thrombomodulin complex. Once activated, PC inactivates two important cofactors of the coagulation
Effect of the scaffold microenvironment on cell polarizability and capacitance determined by probabilistic computations
Betancourt BA, et al.
Bio-medical materials and engineering, 13(2), 025012-025012 (2018)
Thrombin a-chain: activation remnant or allosteric effector?
Carter ISR, et al.
Thrombosis, 2010 (2010)
Platelet subpopulations remain despite strong dual agonist stimulation and can be characterised using a novel six-colour flow cytometry protocol
Sodergren ALand Ramstrom S
Scientific reports, 8(1), 1441-1441 (2018)
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Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

凝血酶因子IIa

凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。

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