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Merck
CN

T7024

四甲基乙二胺

BioReagent, Molecular Biology, ≥99% (GC)

别名:

1,2-双(二甲基氨基)乙烷, TEMED, TMEDA

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关于此项目

线性分子式:
(CH3)2NCH2CH2N(CH3)2
化学文摘社编号:
分子量:
116.20
UNSPSC Code:
41105319
NACRES:
NA.31
PubChem Substance ID:
EC Number:
203-744-6
Beilstein/REAXYS Number:
1732991
MDL number:
Grade:
Molecular Biology
Assay:
≥99% (GC)
Technique(s):
electrophoresis: suitable
Bp:
120-122 °C (lit.)
Vapor pressure:
ca.19.19 hPa
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产品名称

四甲基乙二胺, BioReagent, Molecular Biology, ≥99% (GC)

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

SMILES string

CN(C)CCN(C)C

grade

Molecular Biology

vapor pressure

ca.19.19 hPa

product line

BioReagent

assay

≥99% (GC)

expl. lim.

9.08 %

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

Quality Level

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Application

本产品被指定为分子生物学级,适用于聚丙烯酰胺凝胶电泳(PAGE)。经过分析,不含核酸酶和蛋白酶。
TEMED 是一种叔胺,用于催化过硫酸铵或核黄素形成自由基。TEMED 产生的自由基导致化合物丙烯酰胺和双丙烯酰胺聚合,并形成凝胶基质聚丙烯酰胺。因为 TEMED 仅以其游离碱形式起作用,所以低 pH 条件将抑制聚合。,,
已经研究了 TEMED对银染 SDS PAGE 中背景升高的潜在作用。 TEMED 已经用于在毛细管中涂覆软聚合物(例如聚丙烯酰胺),用于通过原子力显微镜研究。 使用 TEMED 制备含有固定在凝胶垫内的寡核苷酸和蛋白质的微芯片。
通过使用 TEMED 研究了蛋白质的毛细管等电聚焦(等电点(pI)> 12)。 TEMED 已用于小有机分子的 HPLC。

Preparation Note

针对 50mL 丙烯酰胺凝胶溶液,建议用量为 35μL。

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

61.7 °F - closed cup

flash_point_c

16.5 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品
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H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules

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