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Merck
CN

T7527

Tris-硼酸-EDTA缓冲溶液

BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

别名:

TBE 缓冲液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
329826869
UNSPSC Code:
41105319
MDL number:
MFCD00236358

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产品名称

Tris-硼酸-EDTA缓冲溶液, BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioReagent

form

powder blend

impurities

DNase and RNase, none detected

pH

8.1-8.5 (5 ×)

solubility

water: 85.1 g/L, clear, colorless

suitability

suitable for electrophoresis

application(s)

diagnostic assay manufacturing

Quality Level

200

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相关类别

Application

TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
稀释至工作浓度后用于凝胶电泳。

Packaging

1L聚乙烯瓶中所含的粉末混合物可在瓶内溶解并重新配制成 1 L 5倍浓缩液。
4L聚乙烯瓶中所含的粉末混合物可在瓶内溶解并重新配制成 4 L 5 倍浓缩液。


Preparation Note

溶解于指定量的水中得到 5× 浓缩液（0.445M Tris-硼酸、10mM EDTA，pH 为 8.3）。
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000465010
0000465010
0000420656
0000420656
0000366383

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Miyuki Watabe et al.
The Ulster medical journal, 77(3), 168-174 (2008-10-30)
In Northern Ireland over the last 7 years, there is a mean of 41.9 laboratory reports per annum of human gastrointestinal infection (range 19-54) caused by Escherichia coli O157:H7. In the preceding years 1992-1996, reports were 5.4 per annum, whereas
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
Seung-min Park et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15549-15554 (2009-09-01)
Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques

实验方案

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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