一般描述
奇异果甜蛋白是一种从非洲植物卡特莫夫果(Thaumatococcus daniellii Benth)中提取的超甜蛋白质。这种无毒蛋白是广泛的致病相关蛋白家族(PR5组)的成员。奇异果甜蛋白有五种亚型,即奇异果甜蛋白I、II、III、a和b。
应用
来自卡特莫夫果的奇异果甜蛋白用于:
- 作为阳性对照品/标准品,采用蛋白质印迹法检测转基因小鼠乳中奇异果甜蛋白重组蛋白含量
- 作为标准品,比较其与自流汁中出现的葡萄素样蛋白(VVTL1)的保留时间,并测定葡萄汁中的病程相关蛋白含量
- 作为模型蛋白,研究其与游离和连接十钒酸盐的相互作用
生化/生理作用
奇异果甜蛋白是一种零卡路里的甜味剂和风味增强剂,适用于食品工业。它具有抗真菌活性,对植物具有保护作用。奇异果甜蛋白在药剂学中很有用,因为它是一种特殊的低热量膳食成分。
最近有研究表明,翅果竹芋中的奇异果甜蛋白基因在番茄中的过表达导致果实味道变甜,这表明奇异果甜蛋白可用于增强各种食物的味道。
分析说明
奇异果甜蛋白 I 和奇异果甜蛋白 II 的混合物,含有微量的其他甜味蛋白。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
E C Zemanek et al.
Critical reviews in food science and nutrition, 35(5), 455-466 (1995-09-01)
The thaumatins are a class of intensely sweet proteins isolated from the fruit of the tropical plant Thaumatococcus danielli. Thaumatin is approved for use in many countries and has application as both a flavor enhancer and a high-intensity sweetener. The
Tetsuya Masuda et al.
Journal of bioscience and bioengineering, 102(5), 375-389 (2006-12-26)
Most proteins are tasteless and flavorless, while some proteins elicit a sweet-taste response on the human palate. Six proteins, thaumatin, monellin, mabinlin, brazzein, egg lysozyme, and neoculin (previously considered as curculin) have been identified as sweet-tasting proteins. However, no common
Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth.
Bartoszewski, G.
Plant Breeding, 122, 347-351 (2003)
Expression of functional plant sweet protein thaumatin II in the milk of transgenic mice
Lu R, et al.
Food and Bioproducts Processing, 125, 222-227 (2021)
Aleksey Firsov et al.
Protein expression and purification, 123, 1-5 (2016-03-12)
Thaumatin, a supersweet protein from the African plant katemfe (Thaumatococcus daniellii Benth.), is a promising zero-calorie sweetener for use in the food and pharmaceutical industries. Due to limited natural sources of thaumatin, its production using transgenic plants is an advantageous
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