Product Name
3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物体系, peroxidase substrate
质量水平
表单
liquid
储存条件
protect from light
运输
wet ice
储存温度
2-8°C
应用
TMB是辣根过氧化物酶的底物。其可形成可溶性蓝色终产物，并可在370nm或655nm下读数。可以用0.5M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。推荐用于ELISA（微孔）分析，不建议用于膜应用。
通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。
通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。
外形
即用型。
其他说明
免责声明
该底物对光非常敏感，应避免阳光或紫外光源直射。
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1
靶器官
Eyes,Central nervous system
WGK
WGK 2
法规信息
危险化学品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
S Madersbacher et al.
Methods (San Diego, Calif.), 21(1), 41-50 (2000-04-15)
As a glycoprotein hormone, human chorionic gonadotropin (hCG) is not a single molecular entity. This term comprises not only the bioactive heterodimer hCG but also an array of molecular protein backbone and glycosylation variants, such as its free beta (hCGbeta)
Sui-Yuan Chang et al.
Scientific reports, 11(1), 8692-8692 (2021-04-24)
A metal nanoparticle composite, namely TPNT1, which contains Au-NP (1 ppm), Ag-NP (5 ppm), ZnO-NP (60 ppm) and ClO2 (42.5 ppm) in aqueous solution was prepared and characterized by spectroscopy, transmission electron microscopy, dynamic light scattering analysis and potentiometric titration. Based on the in
Wen-Liang Guo et al.
Journal of thoracic disease, 9(2), 287-295 (2017-03-10)
KL-6 is a biomarker of the severity of pulmonary alveolar proteinosis (PAP). We noticed a significant difference in the mean serum KL-6 level between Japanese and Caucasian patients. To assess the clinical value of serum KL-6 in Chinese PAP patients
Eleonora Dehlink et al.
PloS one, 6(4), e19098-e19098 (2011-05-06)
Soluble IgE receptors are potential in vivo modulators of IgE-mediated immune responses and are thus important for our basic understanding of allergic responses. We here characterize a novel soluble version of the IgE-binding alpha-chain of Fc-epsilon-RI (sFcεRI), the high affinity
Janine Wesslowski et al.
The Journal of biological chemistry, 295(26), 8759-8774 (2020-05-10)
The Wingless/Int1 (Wnt) signaling system plays multiple, essential roles in embryonic development, tissue homeostasis, and human diseases. Although many of the underlying signaling mechanisms are becoming clearer, the binding mode, kinetics, and selectivity of 19 mammalian WNTs to their receptors
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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