生物来源
human
质量水平
重组
expressed in NSO cells
方案
>90% (SDS-PAGE)
表单
lyophilized powder
效能
0.1-0.4 μg/mL ED50
分子量
120-130 kDa by SDS-PAGE (reducing)
calculated mol wt 71 kDa
包装
pkg of 100 μg
储存条件
avoid repeated freeze/thaw cycles
技术
western blot: suitable
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
human ... NTRK2(4915)
应用
TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.
生化/生理作用
Binds specifically to mediate neurotrophins BDNF and NT-4/5.
Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.
外形
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.
制备说明
Reconstitute to 100 μg/mL in phosphate buffered saline.
分析说明
Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.
其他说明
Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Di Liu et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 45(9), 1557-1566 (2020-05-20)
Lithium has been used to treat major depressive disorder, yet the neural circuit mechanisms underlying this therapeutic effect remain unknown. Here, we demonstrated that the ventral tegmental area (VTA) dopamine (DA) neurons that project to the medial prefrontal cortex (mPFC)
Marian Tsanov et al.
Hippocampus, 21(1), 1-8 (2010-01-01)
The hippocampus projects to the anterior thalamic nuclei both directly and indirectly via the mammillary bodies, but little is known about the electrophysiological properties of these convergent pathways. Here we demonstrate, for the first time, the presence of long-term plasticity
Yun-yue Ju et al.
Acta pharmacologica Sinica, 36(12), 1437-1443 (2015-11-17)
Brain-derived neurotrophic factor (BDNF) plays an important role in learning and memory in multiple brain areas. In the present study, we investigated the roles of BDNF in aversive memories associated with conditioned drug withdrawal in acute morphine-dependent rats. Conditioned place
Giles S H Yeo et al.
Nature neuroscience, 7(11), 1187-1189 (2004-10-21)
An 8-year-old male with a complex developmental syndrome and severe obesity was heterozygous for a de novo missense mutation resulting in a Y722C substitution in the neurotrophin receptor TrkB. This mutation markedly impaired receptor autophosphorylation and signaling to MAP kinase.
Ana-Clara Bobadilla et al.
Addiction biology, 24(5), 860-873 (2018-06-12)
Brain-derived neurotrophic factor (BDNF) regulates a variety of physiological processes, and several studies have explored the role of BDNF in addiction-related brain regions like the nucleus accumbens core (NAcore). We sought to understand the rapid effects of endogenous BDNF on
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