生物来源
human
质量水平
重组
expressed in NSO cells
方案
>90% (SDS-PAGE)
表单
lyophilized powder
效能
0.1-0.4 μg/mL ED50
分子量
120-130 kDa by SDS-PAGE (reducing)
calculated mol wt 71 kDa
包装
pkg of 100 μg
储存条件
avoid repeated freeze/thaw cycles
技术
western blot: suitable
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
human ... NTRK2(4915)
应用
TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.
生化/生理作用
Binds specifically to mediate neurotrophins BDNF and NT-4/5.
Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.
外形
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.
制备说明
Reconstitute to 100 μg/mL in phosphate buffered saline.
分析说明
Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.
其他说明
Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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