T8768
3,3′,5,5′-四甲基联苯胺 二盐酸盐 水合物
horseradish peroxidase substrate, chromogenic, ≥98%, powder
别名:
TMB 二盐酸盐
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线性分子式:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
化学文摘社编号:
分子量:
313.27 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
产品名称
3,3′,5,5′-四甲基联苯胺 二盐酸盐 水合物, powder, ≥98%
SMILES string
O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI key
KVCWTKDFVVSVSJ-UHFFFAOYSA-N
InChI
1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2
assay
≥98%
form
powder
solubility
acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy
storage temp.
2-8°C
Quality Level
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Application
联苯胺的非致癌性替代品。
3,3′,5,5′-四甲基联苯胺二盐酸盐水合物还用作酶联免疫吸附测定和反向斑点印迹(RDB)分析中的辣根过氧化物酶底物。
General description
3,3′,5,5′-四甲基联苯胺二盐酸盐水合物(TMB-d)是过氧化物酶的敏感显色底物。它没有致癌性，因此可安全替代联苯胺。TMB-d可用于定量血红蛋白和染色粒细胞中含髓过氧化物酶的颗粒。
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Lymphocyte Homing to the Skin: Immunology, Immunopathology, and Therapeutic Perspectives (2004)
Detection of Antibodies Against DNA Polymerase of Hepatitis B Virus in HBsAg-Positive Sera Using ELISA
Rui LX, et al.
The Korean Journal of Internal Medicine, 13, 95-95 (1998)
Robot Printing of Reverse Dot Blot Arrays for Human Mutation Detection
Lappin S, et al.
The Journal of Molecular Diagnostics : JMD, 3, 178-178 (2001)
Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3, 3?, 5, 5?-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine
Liem HH, et al.
Analytical biochemistry, 98(2), 388-393 (1979)
R Piovesana et al.
Scientific reports, 10(1), 7159-7159 (2020-04-30)
Regenerative capability of the peripheral nervous system after injury is enhanced by Schwann cells (SCs) producing several growth factors. The clinical use of SCs in nerve regeneration strategies is hindered by the necessity of removing a healthy nerve to obtain
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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