产品名称
Monoclonal Anti-T7 tag antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
T7 Tag
form
buffered aqueous solution
concentration
~2 mg/mL
technique(s)
western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.
The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
Immunogen
synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH
Physical form
Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Analysis Note
Antibody is suitable for ELISA and immunoblotting.
Application
Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Design of high-throughput methods of protein production for structural biology
Stevens RC
Structure, 8(9), R177-R185 (2000)
Prachi Singh et al.
Medical oncology (Northwood, London, England), 34(8), 145-145 (2017-07-22)
DNA methyltransferases make use of alternative splicing mechanism to generate various splice variants, but their role(s) in modulating DNA methylation patterns in the cells is unclear. Notably, DNMT3B alone contains nearly 40 different splice variants. In this study, we have
Tumor necrosis factor: methods and protocols, 98, 12-12 (2004)
ESSENTIALS OF MEDICINAL CHEMISTRY (2013)
Protein 4.1 R influences myogenin protein stability and skeletal muscle differentiation
Huang SC, et al.
Test, 291(49), 25591-25607 (2016)
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