产品名称
®缓冲剂, BioPerformance Certified, pH 8.3, average Mw 135.4
grade
BioPerformance Certified
form
crystalline powder
mol wt
average Mw 135.4
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)
pH
8.30 (25 °C, 0.1 M)(lit.)
8.3
useful pH range
7.0-9.0
solubility
water: 0.66 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
Quality Level
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Application
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。
Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Other Notes
某些pH电极不能使用Tris缓冲液提供准确的结果。
This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Preparation Note
Trizma溶液可高压灭菌。
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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