T9069
Topoisomerase I human
buffered aqueous glycerol solution, ≥2 units/μL
别名:
DNA Topoisomerase I
recombinant
expressed in baculovirus
form
buffered aqueous glycerol solution
mol wt
100 kDa
concentration
≥2 units/μL
UniProt accession no.
storage temp.
−70°C
Quality Level
Gene Information
human ... TOP1(7150)
General description
Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.
Application
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
Biochem/physiol Actions
Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.
Physical form
Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).
Other Notes
One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
Topoisomerase I in human disease pathogenesis and treatments
Li M and Liu Y
Genomics, Proteomics & Bioinformatics, 14(3), 166-171 (2016)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)
Mechanisms of topoisomerase I (TOP1) gene copy number increase in a stage III colorectal cancer patient cohort
Smith DH, et al.
Testing, 8(4), e60613-e60613 (2013)
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
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