Analysis Note
1 mg 将抑制 ≥0.8 mg 胰蛋白酶，活性约为 10,000 BAEE 单位/mg 蛋白质。
Application
来自莱豆的胰蛋白酶抑制剂 （利马豆）已经被用于：
- 在人血浆中抑制胰蛋白酶
- 在兔骨骼肌中终止蛋白水解消化
- 在来自美洲牧草盲蝽的唾液腺酶提取物的抑制
- 在 龙舌兰 酶提取物中抑制蛋白水解活性
胰蛋白酶抑制剂已被用作分离弹性蛋白酶类酶的亲和配体。
Biochem/physiol Actions
青豆胰蛋白酶抑制剂属于蛋白酶抑制剂的 Bowman-Birk 家族。分子量为 9 kDa，含有 9 个二硫键，热稳定性高。具有胰蛋白酶和糜蛋白酶结合子域。
General description
单体的表观分子量约为 9,000 Da，但发生浓缩和 pH 依赖性二聚化。
Other Notes
在25℃、pH 7.6条件下，反应体积为3.2 mL、光程为1 cm时，以BAEE为底物，一单位胰蛋白酶每分钟可产生0.001的 Δa253 。
查看更多关于 胰蛋白酶抑制剂的信息。
Preparation Note
通过修改 Tauber, H 等人编写的生化与分子生物学行业杂志第 179 期，第 1155 页（1949 年）的方法制备。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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商品
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
实验方案
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
相关内容
QC Methods
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