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Merck
CN

T9525

Tris-硼酸-EDTA缓冲溶液

working solution

别名:

TBE 缓冲液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
41105319
MDL number:
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产品名称

Tris-硼酸-EDTA缓冲溶液, working solution

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

sterility

0.2 μm filtered

form

working solution

technique(s)

electrophoresis: suitable

impurities

DNase, RNase, Protease, none detected

suitability

suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

Quality Level

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Application

TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性(7M 尿素)凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶,但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液,生成的缓冲液含有89mM Tris-硼酸和2mM EDTA,pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液,用来制成PAGE胶。 为了达到最大的分辨率,推荐电压小于5V/cm(电极单元间距离)。

Other Notes

89mM Tris 硼酸,pH 约为 8.3,含有 2mM EDTA。

Packaging

用带龙头分液器包装。

Preparation Note

用 18 兆欧水制备的溶液
采用经生物技术性能认证的 Trizma 碱(产品代码 T6066)、分子生物学试剂硼酸(产品代码 B6768)和 EDTA 二钠盐(产品代码 E5134)制备。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
Seung-min Park et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15549-15554 (2009-09-01)
Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques

实验方案

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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