Merck
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TOX4

Sigma-Aldrich

体外毒理学检测试剂盒,基于中性红

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别名:
NR uptake assay, Neutral red assay
NACRES:
NA.84

形式

liquid

质量水平

用途

 kit sufficient for 1,000 tests

包装

pkg of 1 kit

储存条件

dry at room temperature

λmax

540 nm

应用

cell analysis
detection

检测方法

colorimetric

储存温度

2-8°C

一般描述

用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。

应用

基于中性红的体外毒理检测试剂盒已用于
  • 测定HB8902细胞内溶酶体酶活性
  • 研究人肺泡上皮细胞的细胞活力
  • 研究成纤维细胞的细胞活力

生化/生理作用

中性红(NR)是一种红色染料,通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中,用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。

警示用语:

Danger

危险声明

危险分类

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

73.4 °F

闪点(°C)

23 °C

法规信息

危险化学品

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