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Merck
CN

TOX4

体外毒理学检测试剂盒,基于中性红

别名:

NR uptake assay, Neutral red assay

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关于此项目

NACRES:
NA.84
UNSPSC Code:
12352207
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form

liquid

usage

1 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

540 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

Quality Level

General description

用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。

Application

基于中性红的体外毒理检测试剂盒已用于
  • 测定HB8902细胞内溶酶体酶活性
  • 研究人肺泡上皮细胞的细胞活力
  • 研究成纤维细胞的细胞活力

Biochem/physiol Actions

中性红(NR)是一种红色染料,通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中,用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。

signalword

Danger

Hazard Classifications

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

存储类别

3 - Flammable liquids

wgk

WGK 3

flash_point_f

73.4 °F

flash_point_c

23 °C

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Elisabetta Chieli et al.
Chemico-biological interactions, 186(3), 287-294 (2010-06-02)
Many plant compounds are able to modulate the activity and/or the expression of the major multidrug transporter ABCB1/P-glycoprotein (P-gp). In this study, mango (Mangifera indica L.) stem bark extract (MSBE), its main polyphenol mangiferin and the mangiferin aglycone derivative norathyriol
Jean Yoo et al.
Frontiers in pharmacology, 9, 161-161 (2018-03-16)
Many consumer products used in our daily lives result in inhalation exposure to a variety of chemicals, although the toxicities of the active ingredients are not well known; furthermore, simultaneous exposure to chemical mixtures occurs. Sodium metabisulfite (SM) and propylene
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
An improved colorimetric assay for tumor necrosis factor using WEHI 164 cells cultured on novel microtiter plates.
C D Morgan et al.
Journal of immunological methods, 145(1-2), 259-262 (1991-12-15)
Edward J Perkins et al.
BMC bioinformatics, 7 Suppl 4, S22-S22 (2007-01-16)
Cell culture systems are useful in studying toxicological effects of chemicals such as Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), however little is known as to how accurately isolated cells reflect responses of intact organs. In this work, we compare transcriptional responses in livers of

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