表单
liquid
质量水平
用途
1 kit sufficient for 1,000 tests
包装
pkg of 1 kit
储存条件
dry at room temperature
λmax
540 nm
应用
cell analysis
detection
检测方法
colorimetric
储存温度
2-8°C
一般描述
用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。
应用
基于中性红的体外毒理检测试剂盒已用于：
- 测定HB8902细胞内溶酶体酶活性
- 研究人肺泡上皮细胞的细胞活力
- 研究成纤维细胞的细胞活力
生化/生理作用
中性红(NR)是一种红色染料，通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中，用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。
警示用语：
Danger
危险声明
危险分类
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
73.4 °F
闪点(°C)
23 °C
法规信息
危险化学品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
H Babich et al.
Ecotoxicology and environmental safety, 21(3), 327-336 (1991-06-01)
The PLHC-1 fish hepatoma cell line (Poeciliopsis lucida) was used in the neutral red assay to evaluate the acute cytotoxicities of direct-acting (alkylbenzenes, phthalate diesters, and pesticides) and metabolism-mediated (benzo[a]pyrene) toxicants. The sequence of cytotoxic potencies for the alkylbenzenes and
Thorsten M Seyler et al.
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association, 33(2), 374-386 (2016-10-04)
To evaluate the biological, immunological, and biomechanical properties of a scaffold derived by architectural modification of a fresh-frozen porcine patella tendon using a decellularization protocol that combines physical, chemical, and enzymatic modalities. Porcine patellar tendons were processed using a decellularization
Adam L Nathan et al.
Journal of medical devices, 11(1), 0110091-110099 (2017-02-10)
Highly porous, open-celled shape memory polymer (SMP) foams are being developed for a number of vascular occlusion devices. Applications include abdominal aortic and neurovascular aneurysm or peripheral vascular occlusion. A major concern with implanting these high surface area materials in
Simonetta Palleschi et al.
Chemosphere, 196, 35-44 (2018-01-01)
Fine airborne particulate matter (PM2.5) has been repeatedly associated with adverse health effects in humans. The PM2.5 soluble fraction, and soluble metals in particular, are thought to cause lung damage. Literature data, however, are not consistent and the role of
An improved colorimetric assay for tumor necrosis factor using WEHI 164 cells cultured on novel microtiter plates.
C D Morgan et al.
Journal of immunological methods, 145(1-2), 259-262 (1991-12-15)
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