form
liquid
usage
1 kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
540 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
2-8°C
Quality Level
General description
用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。
Application
基于中性红的体外毒理检测试剂盒已用于：
- 测定HB8902细胞内溶酶体酶活性
- 研究人肺泡上皮细胞的细胞活力
- 研究成纤维细胞的细胞活力
Biochem/physiol Actions
中性红(NR)是一种红色染料，通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中，用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
73.4 °F
flash_point_c
23 °C
法规信息
危险化学品
此项目有
Elisabetta Chieli et al.
Chemico-biological interactions, 186(3), 287-294 (2010-06-02)
Many plant compounds are able to modulate the activity and/or the expression of the major multidrug transporter ABCB1/P-glycoprotein (P-gp). In this study, mango (Mangifera indica L.) stem bark extract (MSBE), its main polyphenol mangiferin and the mangiferin aglycone derivative norathyriol
Jean Yoo et al.
Frontiers in pharmacology, 9, 161-161 (2018-03-16)
Many consumer products used in our daily lives result in inhalation exposure to a variety of chemicals, although the toxicities of the active ingredients are not well known; furthermore, simultaneous exposure to chemical mixtures occurs. Sodium metabisulfite (SM) and propylene
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
An improved colorimetric assay for tumor necrosis factor using WEHI 164 cells cultured on novel microtiter plates.
C D Morgan et al.
Journal of immunological methods, 145(1-2), 259-262 (1991-12-15)
Edward J Perkins et al.
BMC bioinformatics, 7 Suppl 4, S22-S22 (2007-01-16)
Cell culture systems are useful in studying toxicological effects of chemicals such as Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), however little is known as to how accurately isolated cells reflect responses of intact organs. In this work, we compare transcriptional responses in livers of
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