Merck
CN

TP0010

Millipore

FLAG ® HA 串联亲和纯化试剂盒

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别名:
抗 ddddk, 抗 dykddddk
NACRES:
NA.32

一般描述

TAP 技术是蛋白质纯化的最新进展。该技术将串联的亲和标签整合到感兴趣的基因中,从而可以通过两个连续的亲和纯化步骤分离出高纯度的融合蛋白。该技术特别适用于通过向已知的“诱饵”蛋白添加串联亲和标签来分离和鉴定蛋白质复合物,以减少与目标靶蛋白相互作用的内源蛋白质。

应用

FLAG ® HA 串联亲和纯化试剂盒专门设计用于从复杂基质(如细胞裂解物和组织匀浆)中分离高纯度 FLAG-HA 双标签融合蛋白。该试剂盒特别适用于使用 TAP(串联亲和纯化)技术分离蛋白复合物。

可用于质谱分析和免疫印迹。

在我们的 FLAG ®Literature 门户网站可浏览其他应用参考信息。

法律信息

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • F2426EZview Red ANTI-FLAG® M2 Affinity Gel, clone M2化学品安全说明书

  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension化学品安全说明书

  • R0278RIPA Buffer化学品安全说明书

  • F47993X FLAG® Peptide, lyophilized powder化学品安全说明书

  • U4883Urea, 8 M (after reconstitution with 16 mL high purity water)化学品安全说明书

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危险声明

危险分类

Aquatic Chronic 2 - Eye Irrit. 2

储存分类代码

10 - Combustible liquids

法规信息

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Wei Li et al.
Cell, 140(4), 477-490 (2010-02-25)
Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth-inhibitory form of Merlin accumulates in the nucleus, binds to
Junsong Zhang et al.
Journal of virology, 90(8), 3966-3980 (2016-02-05)
The viral ribonucleoprotein (vRNP) complex of influenza A viruses (IAVs) contains an RNA-dependent RNA polymerase complex (RdRp) and nucleoprotein (NP) and is the functional unit for viral RNA transcription and replication. The vRNP complex is an important determinant of virus
Karthik Ramachandran et al.
iScience, 25(1), 103722-103722 (2022-01-11)
SARS-CoV-2 is a newly identified coronavirus that causes the respiratory disease called coronavirus disease 2019 (COVID-19). With an urgent need for therapeutics, we lack a full understanding of the molecular basis of SARS-CoV-2-induced cellular damage and disease progression. Here, we
Kosei Sato et al.
Nature communications, 10(1), 166-166 (2019-01-13)
In Drosophila, some neurons develop sex-specific neurites that contribute to dimorphic circuits for sex-specific behavior. As opposed to the idea that the sexual dichotomy in transcriptional profiles produced by a sex-specific factor underlies such sex differences, we discovered that the

商品

The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.

相关内容

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Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

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