用途
sufficient for ~200 assays
质量水平
一般描述
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
应用
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.
生化/生理作用
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
特点和优势
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
8A - Combustible corrosive hazardous materials
WGK
WGK 3
法规信息
新产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Expression of alpha-gustducin and alpha-transducin, G proteins coupled with taste receptors, in boar sperm
Spinaci M, et al.
Theriogenology, 82(1), 144-151 (2014)
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive
Dalia A Elgamal et al.
Molecular and cellular endocrinology, 503, 110700-110700 (2020-01-07)
Genetic and epigenetic factors contribute equally to the pathogenesis of type 1 diabetes mellitus. Sodium butyrate (NaB) has been reported to improve glucose homeostasis by modulation of the p38/ERK MAPK pathway. This work aims to evaluate the effect of NaB
Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress
Bernardini C, et al.
Cell Stress & Chaperones, 15(2), 219-224 (2010)
Chiara Bernardini et al.
Journal of inflammation (London, England), 9(1), 47-47 (2012-11-28)
It is well known that nitric oxide (NO) is generated by a family of constitutively (nNOS and eNOS) or inducibly (iNOS) expressed enzymes and takes part in different aspects of the inflammatory response; nevertheless, its effective role in the pathogenesis
实验方案
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
相关内容
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
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