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Merck
CN

TRUE2

Sigma-Aldrich

TrueGel3D 水凝胶试剂盒

FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.71
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运输

dry ice

储存温度

−70°C

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一般描述

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on PEG non cell-degradable crosslinker. Maleimide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD (TRUERGD-1UM) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

应用

TRUE2-1KT is used to grow cells that don′t attach to hydrogel for survival but rather use their secreted extracellular matrix. It is used to immobilize cells or specimens for imaging and for bioprinting.

特点和优势

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)
  • Does not allow cell migration

其他说明

TRUE2-1KT contains lyophilized FAST-DEXTRAN (polymer) and PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol, and water.

免责声明

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

水凝胶是最常用的 3D 细胞培养系统。了解关于该技术的更多信息(什么是水凝胶?如何使用水凝胶?)

查看所有结果

实验方案

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

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