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Merck
CN

TRUE9

Sigma-Aldrich

TrueGel3D 水凝胶试剂盒

SLO-PVA, PEG non cell-degradable crosslinker

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.71

主要文件

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运输

dry ice

储存温度

−70°C

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相关类别

一般描述

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

应用

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

特点和优势

  • Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Does not allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

其他说明

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

免责声明

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290,H315,H319

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible corrosive hazardous materials

WGK

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0001622521
0001615167
0006165167

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Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects
Ferreira LP, et al.
Acta Biomaterialia (2018)

商品

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

3D细胞培养水凝胶

水凝胶是最常用的 3D 细胞培养系统。了解关于该技术的更多信息（什么是水凝胶？如何使用水凝胶？）

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实验方案

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

相关内容

3D Cell Culture Workflow Tools

Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.

Product Information Sheet - TRUE9

Instructions

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