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Merck
CN

TRUEDEXS

Sigma-Aldrich

TrueGel3D聚合物

Modified for slow gelling (SLO-DEXTRAN), allow cell recovery

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.71
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技术

cell attachment: suitable

运输

dry ice

储存温度

−70°C

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一般描述

Dextrose polymers are functionalized with thiol-reactive groups, which react at a slower rate with thiol groups of crosslinkers (CD/PEG crosslinkers) to form stable hydrogels. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of homogenous gel formation. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

应用

SLO-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

特点和优势

  • Contains dextrose polymer functionalized with thiol reactive groups for slow gelation
  • Degradable polymer

其他说明

TRUEDEXS contains SLO-DEXTRAN solution in phosphate buffer, TrueGel3D thioglycerol and water

免责声明

Avoid frequent freeze-thaw cycles and store SLO-DEXTRAN in small portions

象形图

Corrosion

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible corrosive hazardous materials

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物(例如小鼠EHS肿瘤细胞)的水凝胶相比,TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

实验方案

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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