form
lyophilized powder
packaging
pkg of 2500 units, pkg of 500 units, pkg of One unit of ThermaStop™ is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
technique(s)
PCR: suitable
color
opaque
shipped in
ambient
storage temp.
2-8°C
General description
ThermaStop™ 是一种可逆的热启动试剂，与热稳定的DNA聚合酶相容。它能直接作用于聚合酶，以防止低于50℃时的非特异性酶活性。聚合酶活性在60℃时完全恢复，但通过冷却反应ThermaStop™ 会再次被抑制。 将ThermaStop™ 添加到您的PCR反应中可以提高灵敏度和总产物产量。
Application
ThermaStop™ 已被用作PCR预混液的成分，用于通过非多重巢式PCR进行人类免疫缺陷病毒（HIV-1）的前病毒基因组扩增。 它也被用于HIV感染者口腔拭子中DNA的扩增。
Features and Benefits
- 将ThermaStop™ 添加到您的PCR反应中可以提高灵敏度和总产物产量
- 增加产物产量以用于下游应用，如基因分型、下一代测序（NGS）样品制备、Gibson assembly、诊断和多重PCR
- 它改善聚合酶特异性，增进PCR性能和定量数据
- 防止在PCR以前形成引物-二聚体和非特异性产物
- 使预混液（master mix）在PCR以前可在室温下稳定36小时
- 在第一个PCR周期期间快速失活
- 冷却时可快速重新激活
Preparation Note
溶于Tris pH 8.3缓冲液中
Legal Information
ThermaStop is a trademark of ThermaGenix Inc.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Jan Clement Santiago et al.
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Basiel Cole et al.
Nature communications, 12(1), 3727-3727 (2021-06-19)
Clonal expansion of HIV-infected cells contributes to the long-term persistence of the HIV reservoir in ART-suppressed individuals. However, the contribution from cell clones that harbor inducible proviruses to plasma viremia is poorly understood. Here, we describe a single-cell approach to
商品
How to stop nonspecific bands and off target primers in PCR.
PCR additive added to master mix that stops nonspecific bands after cool down.
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
ThermaStop™ 和 ThermaGo™ PCR 添加剂可抑制扩增前、扩增中和扩增后的错配，从而提高PCR的特异性和灵敏度。ThermaStop™-RT添加剂在一步法和两步法RT-PCR中可作为逆转录酶的热启动剂。
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