TSTOPRT
ThermaStop™-RT
increases RT-PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before RT-PCR
别名:
Multiplex RT-PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
form
lyophilized powder
packaging
pkg of 2500 units, pkg of 500 units
technique(s)
RT-PCR: suitable
color
opaque
shipped in
ambient
storage temp.
2-8°C
General description
ThermaStop™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.
Application
ThermaStop™-RT is suitable for gene expression quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.
Features and Benefits
- It eliminates the non-specific product formation
- Provides increased sensitivity and specificity
- Provides higher yield and pure cDNA synthesis for conventional RT-PCR
- It minimizes the primer-dimer formation
- It utilizes the same cycling conditions as conventional Taq polymerase
- Decreases the RNA secondary structure
- Stable at low temperatures
- Enzymes are inhibited at below 55°C
- Compatible with several RT-PCR enzymes
- Provides better multiplexing, efficient primer use
- Better quantitative accuracy and monoplex and multiplex amplification sensitivity
Packaging
One unit of ThermaStop™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 uL and a temperature of 50 degrees C.
Preparation Note
Dissolve in Tris pH 8.3 buffer
Other Notes
RT-PCR, *Please note ThermaStop™-RT should not be used in combination with ThermaStop™ or ThermaGo™.
Legal Information
ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.
wgk
WGK 3
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
How to stop nonspecific bands and off target primers in PCR.
PCR additive added to master mix that stops nonspecific bands after cool down.
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
ThermaStop™ 和 ThermaGo™ PCR 添加剂可抑制扩增前、扩增中和扩增后的错配，从而提高PCR的特异性和灵敏度。ThermaStop™-RT添加剂在一步法和两步法RT-PCR中可作为逆转录酶的热启动剂。
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