TT0010
Trypsin Spin Columns
Utilizes TPCK-treated porcine trypsin immobilized on a 20 µm silica support, ensuring minimal chymotryptic activity, suitable for mass spectrometry
别名:
Immobilized TPCK- treated Trypsin, Immobilized Trypsin
产品名称
Trypsin Spin Columns,
储存温度
2-8°C
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一般描述
Enjoy all of the advantages of Proteomics Grade Trypsin in a 15-minute digestion. A staple of MS sample preparation, efficient tryptic digestion is essential to successful proteomic analyses. While traditional digests require up to 18 hours, the same digest can be accomplished in only 15 minutes using the Trypsin Spin Column, Proteomics Grade.
This ultra-micro spin column contains highly purified, TPCK-treated porcine trypsin immobilized on a spherical 20 micron silica support, chemically modified to minimize non-specific adsorption. This product is ideal for rapid protein digestion of small volumes (100 μl or less). Eluted peptides are ready for MS analysis, and require no additional clean-up.
This ultra-micro spin column contains highly purified, TPCK-treated porcine trypsin immobilized on a spherical 20 micron silica support, chemically modified to minimize non-specific adsorption. This product is ideal for rapid protein digestion of small volumes (100 μl or less). Eluted peptides are ready for MS analysis, and require no additional clean-up.
特点和优势
Discover the advantages for yourself!
- 15 minute digestion
- Ideal for 10 to 100 μg of protein sample
- Minimal chymotryptic activity
- No additional clean-up required
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
此项目有
Jiangjiang Liu et al.
Journal of the American Society for Mass Spectrometry, 20(5), 819-828 (2009-02-10)
This work uses electrospray ionization mass spectrometry (ESI-MS) in conjunction with hydrogen/deuterium exchange (HDX) and optical spectroscopy for characterizing the solution-phase properties of cytochrome c (cyt c) after heat exposure. Previous work demonstrated that heating results in irreversible denaturation for
Shama P Mirza et al.
Journal of proteome research, 7(7), 3042-3048 (2008-05-31)
Proteomics-based quantification methods for differential protein expression measurements are among the most important and challenging techniques in the field of mass spectrometry. Though numerous quantification methods have been established, no method meets all the demands for measuring accurate protein expression
Logan J Everett et al.
Journal of proteome research, 9(2), 700-707 (2009-12-02)
"Multi-stage" search strategies have become widely accepted for peptide identification and are implemented in a number of available software packages. We describe limitations of these strategies for validation and decoy-based statistical analyses and demonstrate these limitations using a set of
Tomoharu Takeuchi et al.
Biological & pharmaceutical bulletin, 34(7), 1139-1142 (2011-07-02)
To study the endogenous counterpart of LEC-6, a major galectin in Caenorhabditis elegans, the proteomic analysis of glycoproteins captured by an immobilized LEC-6 column was performed using the nano liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. A protein recovered in a
Hans W Hahn et al.
Journal of proteome research, 8(9), 4225-4230 (2009-07-31)
Trypsin was immobilized on glycidylmethacrylate-co-divinylbenzene (GMA/DVB) polymerized in pipet tips for online enzymatic digestion of proteins. The major advantages of in-tip digestion are easy handling and small sample amount required for analysis. Microwave-assisted digestion was applied for highly efficient and
相关内容
High Density Immobilized Trypsin in a Spin Column Format for Rapid Proteolysis and Increased Sequence Coverage
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