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Merck
CN

U0251

尿素酶 来源于洋刀豆 (刀豆)

Type C-3, powder, ≥600,000 units/g solid

别名:

Jack bean urease, Urea amidohydrolase

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
≥600,000 units/g solid
Biological source:
Canavalia ensiformis
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biological source

Canavalia ensiformis

Quality Level

type

Type C-3

form

powder

specific activity

≥600,000 units/g solid

mol wt

~544620 Da

purified by

crystallization

storage temp.

−20°C

General description

亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
尿素酶是一种依赖镍的金属酶,存在于各种植物、细菌、真菌和藻类中。它含有两个不同的亚基——UreA(26.5 kDa)和UreB(60.3–61.6 kDa)。

Biochem/physiol Actions

在 Hela 细胞中,脲酶可诱导空泡化,这种作用取决于脲酶将尿素转化为氨的能力。
尿素酶有助于植物中氮的生物利用。它与防御机制有关,具有杀虫活性。此外,尿素酶可通过抑制丝状真菌和酵母的生长和影响细胞膜完整性表现出抗真菌活性。

Other Notes

一微摩尔单位,在 pH7.0,25℃ 下,每分钟从尿素中释放出 1.0 μmole 的 NH3。它相当于1.0 I.U. 或 0.054 Sumner 单位(在 pH 7.0,20°C,5 分钟内 1.0 mg 氨氮)


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pictograms

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signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

法规信息

低风险生物材料

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Barbara Krajewska et al.
Plant physiology and biochemistry : PPB, 43(7), 651-658 (2005-07-19)
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in
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