U0251
尿素酶 来源于洋刀豆 （刀豆）
Type C-3, powder, ≥600,000 units/g solid
别名:
Jack bean urease, Urea amidohydrolase
一般描述
亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
由六个亚基组成，总分子量：约 544620
尿素酶是一种依赖镍的金属酶，存在于各种植物、细菌、真菌和藻类中。它含有两个不同的亚基——UreA（26.5 kDa）和UreB（60.3–61.6 kDa）。
生化/生理作用
在 Hela 细胞中，脲酶可诱导空泡化，这种作用取决于脲酶将尿素转化为氨的能力。
尿素酶有助于植物中氮的生物利用。它与防御机制有关，具有杀虫活性。此外，尿素酶可通过抑制丝状真菌和酵母的生长和影响细胞膜完整性表现出抗真菌活性。
其他说明
一微摩尔单位，在 pH7.0，25℃ 下，每分钟从尿素中释放出 1.0 μmole 的 NH3。它相当于1.0 I.U. 或 0.054 Sumner 单位（在 pH 7.0，20°C，5 分钟内 1.0 mg 氨氮）
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Barbara Krajewska et al.
Plant physiology and biochemistry : PPB, 43(7), 651-658 (2005-07-19)
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in
T L Cover et al.
Infection and immunity, 59(4), 1264-1270 (1991-04-01)
Concentrated broth culture supernatants from 50 to 60% of Helicobacter pylori strains induce eukaryotic cell vacuolation in vitro. A quantitative assay for cell vacuolation was developed on the basis of the rapid uptake of visibly vacuolated HeLa cells was significantly
J Ryan Feathers et al.
Journal of structural biology: X, 5, 100047-100047 (2021-04-06)
The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit
J Jessy et al.
The Biochemical journal, 277 ( Pt 3), 693-696 (1991-08-01)
Recent studies showed that hyperammonaemia caused many of the metabolic changes in portacaval-shunted rats, a model of hepatic encephalopathy. These changes included a depression in the cerebral metabolic rate of glucose (CMRGlc), an indication of decreased brain function. 2. The
N E Dixon et al.
Science (New York, N.Y.), 191(4232), 1144-1150 (1976-03-19)
In an attempt to understand the role of nickel in jack bean urease (1), we turned to a variety of other enzymes important in the utilization, production, or transfer of ammonia. We found several, including the L-histidine and L-phenylalanine ammonialyases
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