U1875
尿素酶 来源于洋刀豆 （刀豆）
Type III, glycerol solution, 500-800 units/mL
别名:
Jack bean urease, Urea amidohydrolase
Application
来自直生刀豆（洋刀豆）的尿素酶已用于：
- 使用从左心室（LV）和室间隔组织中提取的样本测定肌酸和磷酸肌酸
- 测定稳定尿样中尿素的浓度
- 一种标准品，以测定植物提取物的尿素酶活性
Biochem/physiol Actions
在植物中，尿素酶对于自然界中氮的生物利用度/代谢和防御机制至关重要。尿素酶是一种用于催化尿素水解的金属酶，以释放生长所需氮源—氨以及二氧化碳。他们表现内部肽介导的杀虫作用和杀菌活性。
脲酶活性已显示在暴露于光后被苯并芘抑制。这种抑制作用是由于苯并芘对脲酶′巯基的作用。
General description
亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
由六个亚基组成，总分子量：约 544620
洋刀豆（直生刀豆）的尿素酶是一种含有840个氨基酸残基的单链六聚体，存在于植物、细菌、真菌、藻类和土壤中。它被视为是第一种含镍的酶，也是第一种结晶化酶。尿素酶以多种异构体存在，部分包括洋刀豆尿素酶（JBURE-I）、刀豆毒素（CNTX）和JBURE-IIB。JBURE-I比其他两种异构体更加丰富。尿素酶存在成熟的种子中。
Other Notes
一微摩尔单位是指在pH 7.0，25℃下每分钟催化尿素释放1.0 μmole NH3所需的酶活性。它相当于1.0 I.U.或0.054 Sumner单位（在pH 7.0，20℃下，5分钟内产生1.0 mg氨氮）
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
此项目有
Fernando Medina Ferrer et al.
Microbial biotechnology, 13(6), 1877-1888 (2020-07-29)
Microbial precipitation of calcium carbonate is a widespread environmental phenomenon that has diverse engineering applications, from building and soil restoration to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate
Melissa Postal et al.
Peptides, 38(1), 22-32 (2012-08-28)
Ureases (EC 3.5.1.5) are metalloenzymes that hydrolyze urea into ammonia and CO(2). These proteins have insecticidal and fungicidal effects not related to their enzymatic activity. The insecticidal activity of urease is mostly dependent on the release of internal peptides after
Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Fernando Medina Ferrer et al.
Geobiology, 20(1), 79-97 (2021-08-03)
Modern carbonate tufa towers in the alkaline (~pH 9.5) Big Soda Lake (BSL), Nevada, exhibit rapid precipitation rates (exceeding 3 cm/year) and host diverse microbial communities. Geochemical indicators reveal that carbonate precipitation is, in part, promoted by the mixing of calcium-rich
Tomoyo Matsushita Yamamoto et al.
Medical molecular morphology, 45(4), 199-205 (2012-12-12)
Electrolyzed acid water (EAW) has been studied for its disinfective potential against pathogenic microbes; however, the bactericidal process against Mycobacteria has not been clearly presented. In this study, to clarify the disinfective process against Mycobacteria, EAW-treated bacteria were examined against
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