Merck
CN

U211140

Sigma-Aldrich

DMT-2′O-Methyl-rU Phosphoramidite

configured for MerMade

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别名:
DMT-2′O-甲基-rU酰胺
经验公式(希尔记法):
C40H49N4O9P
分子量:
760.81
MDL编号:
NACRES:
NA.51

生物来源

non-animal source (no BSE/TSE risk)

质量水平

产品线

Proligo Reagents

检测方案

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)

形式

powder

分子量

760.81 g/mol

杂质

≤0.3% mU2 (reversed phase HPLC, Hydrolysate)
≤0.3% mU3 (reversed phase HPLC, DMT-rme)
≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (redox titration)
≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT)
≤3% residual Solvent content

颜色

white to off-white

适用性

conforms to structure for H-NMR
conforms to structure for LC-MS

相容性

configured for MerMade

储存温度

2-8°C

SMILES字符串

CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1N5C=CC(=O)NC5=O

InChI

1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1

InChI key

UVUOJOLPNDCIHL-XKZJCBTISA-N

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一般描述

DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites. Its key features include:
  • High yield of crude oligonucleotides Compatible with DNA synthesis
  • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
  • Recommended deprotection conditions are 8 hours at 55 deg C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 deg C
  • Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
  • Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers)
  • 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry

应用

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

  • Diagnostic probes
  • Aptamer and ribozyme development
  • Mixed 2′O-Methyl-RNA/DNA antisense molecules

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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