U7251
Uridine-5′-diphosphoglucose Dehydrogenase from bovine liver
Type VI, lyophilized powder, ≥0.1 units/mg protein
别名:
UDP-glucose:NAD+ 6-oxidoreductase
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-826-4
MDL number:
type
Type VI
form
lyophilized powder
specific activity
≥0.1 units/mg protein
composition
Protein, 30-60%
storage temp.
−20°C
Biochem/physiol Actions
UDP-glucose 6-dehydrogenase, also known as uridine-5′-diphosphoglucose dehydrogenase, was shown to bind NAD and NADPH at a saturated amount of 0.5 mol and 2 mol respectively. The binding of UDP-glucose 6-dehydrogenase to NADPH was pH-dependent.
Physical form
Lyophilized powder containing ~30% potassium phosphate and ~20% sodium citrate
Analysis Note
Protein determined by Modified Warburg-Christian method.
Other Notes
One unit will oxidize 1.0 μmole of UDP-glucose to UDP-glucuronic acid per min at pH 8.7 at 25 °C.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
相关内容
Instructions
Shahid Siddique et al.
PloS one, 7(7), e41515-e41515 (2012-08-01)
The cyst nematode Heterodera schachtii infects roots of Arabidopsis plants and establishes feeding sites called syncytia, which are the only nutrient source for nematodes. Development of syncytia is accompanied by changes in cell wall structures including the development of cell
P A Gainey et al.
The Biochemical journal, 141(3), 667-673 (1974-09-01)
The binding of NAD(+) and NADH to bovine liver UDP-glucose dehydrogenase was studied by using gel-filtration and fluorescence-titration methods. The enzyme bound 0.5mol of NAD(+) and 2 mol of NADH/mol of subunit at saturating concentrations of both substrate and product.
Takenori Satomura et al.
Bioscience, biotechnology, and biochemistry, 75(10), 2049-2051 (2011-10-08)
A gene encoding a UDP-glucose dehydrogenase homologue was identified in the hyperthermophilic archaeon, Pyrobaculum islandicum. This gene was expressed in Escherichia coli, and the product was purified and characterized. The expressed enzyme is the most thermostable UDP-glucose dehydrogenase so far