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化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-811-2
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
Biological source:
bakers yeast
biological source
bakers yeast
type
Type X
form
lyophilized powder
specific activity
≥40 units/mg protein
composition
Protein, 30-60% modified Warburg-Christian
foreign activity
UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%, inorganic pyrophosphatase ≤0.5%
storage temp.
−20°C
Quality Level
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General description
尿苷-5′-二磷酸葡萄糖焦磷酸化酶(UDP-glc-PPase)普遍存在于植物、酵母、细菌和哺乳动物中。这种酶是由八个相同亚基组成的八聚体。
Application
来自面包酵母的尿苷-5′-二磷酸葡萄糖焦磷酸化酶′已用于:
- 合成尿苷-5′-二磷酸葡萄糖(UDP-Glc)-13C9
- 量化水稻中蔗糖合酶(SUS)的活性
- 研究己糖激酶和糖原合成酶控制青蛙卵母细胞通量的作用
Biochem/physiol Actions
尿苷-5′-二磷酸葡萄糖焦磷酸化酶(UDP-glc-PPase)参与催化UDP-葡萄糖的合成。这种酶的活性需要二价阳离子,例如Mg2+、Ca2+、Mn2+、Ni2+。
Physical form
冻干粉,不含硫酸盐,含柠檬酸缓冲盐
Other Notes
一个单位将在25℃和pH 7.6下,每分钟由尿苷-5′-二磷酸葡萄糖和无机焦磷酸盐形成1.0 μmole的1-磷酸葡萄糖。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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Michael L N, et al.
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Kato T, et al.
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Bacterial galU coding for a uridine diphosphate-glucose pyrophosphorylase plays an important role in carbohydrates biosynthesis, including synthesis of lipopolysaccharides (LPS), membrane-derived oligosaccharides, and capsular polysaccharides. In this study, we characterized the galU mutant of Pseudomonas syringae pv. syringae 61 (Psy61)
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