V4888
抗 VSV-G 兔抗
affinity isolated antibody, buffered aqueous solution
别名:
抗 水泡性口炎病毒糖蛋白
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
73
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mammals
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells, western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
抗 VSV-G 兔抗体与氨基端和羧基端 VSV-G 标记的融合蛋白反应。
水泡性口炎病毒（VSV）属于弹状病毒科（Rhabdoviridae）的水泡病毒属（Vesiculovirus）。水泡性口炎病毒G（VSV G）蛋白被认为是早期鉴定出的III型病毒融合蛋白。
Immunogen
与水疱性口炎病毒糖蛋白 (VSV-G) 的氨基酸 497-511 相应的 N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C）相对应的合成肽，通过在羧基端添加半胱氨酸残基与马来酰亚胺结合活化 KLH。该抗体可识别 C 端和 N 端的 VSV-G 标签。
Application
兔生产的抗VSV-G 抗体已被用于免疫印迹和免疫沉淀。
抗体适用于免疫印迹、免疫沉淀和间接免疫荧光。
免疫组化。
免疫组化。
Biochem/physiol Actions
在低pH刺激下，水泡性口炎病毒G（VSV G）能够刺激细胞融合。VSV G蛋白的跨膜区对于病毒从半融合状态向完全融合状态的转变至关重要。
Physical form
溶于含有1%牛血清白蛋白和15mM叠氮化钠（作为防腐剂）的0.01M磷酸盐缓冲盐水（pH7.4）中。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
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Martinez I, et al.
Journal of virology, 77(14), 8039-8047 (2003)
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