VEGF Receptor-1 (Flt-1)/Fc Chimera from mouse

VEGF Receptor-1 (Flt-1)/Fc Chimera from mouse was used for investigating the vascular potential of hematopoietic stem cells.[1] It may be used to study vasculogenesis and angiogenesis.

VEGF R1 binds VEGF with high affinity and is a potent VEGF inhibitor.

VEGF Receptor-1 (VEGF R1) also known as Flt-1 belongs to class III subfamily of RTKs and expresses mainly in endothelial cells. VEGF R1 binds to vascular endothelial growth factor B (VEGF-B) and hence facilitates in regulating the plasminogen activator activity in endothelial cells.[2]It also assists endothelial cells in the proper spatial organization of lumen-containing vessels. Additionally, alternatively spliced VEGF R1 pre-mRNA helps in regulating the VEGF activity in angiogenesis.[3]

VEGF receptors, class III receptor tyrosine kinases, have seven Ig like extracellular motifs and a tyrosine kinase intracellular domain split by a kinase insert sequence. VEGFR 1 was originally called fms like tyrosine kinase 1 (flt 1), indicating its homology to receptors of PDGF, M CSF, and SCF. VEGFR 1 is one of the five receptor tyrosine kinases (KDR/Flk-1, Flt-4, tie-1 and tek/tie-2) whose expression is almost exclusively restricted to endothelial cells. These receptors are likely to play central roles in vasculogenesis and angiogenesis. Recombinant VEGF R1 binds VEGF with high affinity and is a potent VEGF inhibitor. VEGFR 1 and 2 are both expressed in an endothelial cell-specific manner and are detectable in virtually all tissues in adults and embryos. Hypoxia induces endothelial cell expression of VEGFR 1 but not VEGFR 2. Monocytes express VEGFR 1 and 2 and can migrate to the site of VEGF release, but this response appears to be VEGFR 1 mediated. VEGFR 2 is also expressed in pancreatic duct cells. VEGFR 2 is involved in commitment of endothelial-cell lineages and to cell proliferation, while VEGFR 1 seems to be responsible for guiding endothelial cells into the proper spatial organization into lumen-containing vessels. VEGFR 2 is a key marker for pluripotent HSCs. Neurophilins, a group of unrelated receptors for some VEGF family members may also act as coreceptors to facilitate VEGF binding to VEGFR 2. VEGFR 3 is a specific marker for lymphatic vessels. VEGFR 3 has also been detected on some high endothelial venules, in embryonic pre-lymphatic blood vessels, some tumor blood vessels, and certain hematopoietic and leukemia cells.

Lyophilized from a 0.2 μm filitered solution in 20 mM MOPS, 500 mM NaCl and 0.05% CHAPS

The biological activity is measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells.