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Merck
CN

W1878

SAFC

Williams′ 培养基E

With sodium bicarbonate, without ʟ-glutamine and phenol red, sterile liquid, suitable for cell culture

Pharma Manufacturing

别名:

Williams’ E medium

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

product name

Williams′ 培养基E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

运输

ambient

储存温度

2-8°C

一般描述

Williams培养基E由Williams和Gunn在Williams培养基D的基础上改良而成。培养基专用于支持成人肝脏上皮细胞的长期细胞培养。它是改良型最低必需培养基(MEM),改变了葡萄糖和氨基酸的含量。

应用

Williams培养基E已用于培养人肝细胞癌(HCC)细胞和肝细胞。

重悬

添加 0.292g/L L-谷氨酰胺。

其他说明

酚红会干扰一些细胞在低密度或克隆密度下的生长。在使用干细胞或低密度细胞时,请使用这款William's E。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Balakrishnan Chakrapani Narmada et al.
Stem cells translational medicine, 6(3), 851-863 (2017-03-16)
Atherosclerosis underlies many cardiovascular and cerebrovascular diseases. Nutraceuticals are emerging as a therapeutic moiety for restoring vascular health. Unlike small-molecule drugs, the complexity of ingredients in nutraceuticals often confounds evaluation of their efficacy in preclinical evaluation. It is recognized that
Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
Ezgi Eyluel Bankoglu et al.
PloS one, 11(11), e0166956-e0166956 (2016-11-29)
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in

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