Quality Segment
form
liquid
feature
Direct PCR
storage condition
OK to freeze (-10 to-25°C)
greener alternative product characteristics
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RT-qPCR: suitable, qPCR: suitable
suitability
suitable for PCR (suitable for RT-qPCR)
greener alternative category
shipped in
dry ice
storage temp.
−20°C
General description
The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.
We are committed to bringing you Greener Alternative Products, which belongs to one of the four categories of greener alternatives. This product is a Design for Sustainability (DfS) developed product. Click here to view its DfS scorecard.
We are committed to bringing you Greener Alternative Products, which belongs to one of the four categories of greener alternatives. This product is a Design for Sustainability (DfS) developed product. Click here to view its DfS scorecard.
Application
- The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
- qRT-PCR
- RT-LAMP
- NGS library preparation
Features and Benefits
- From cell lysis to qRT-PCR in 15 minutes
- High Throughput and automation-friendly
- No RNA purification or clean up necessary
- No columns, heat, or centrifugation necessary
- Compatible with both adherent and non-adherent mammalian cell types
- Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes
Physical form
Liquid
Preparation Note
Freezer (-10 to-25°C)
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Extract-N-AMP Cellular RNA Lysis buffer 5ML, 50ML
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 2