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XNAP2

Sigma-Aldrich

Extract-N-Amp™植物PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

Plant direct PCR kit

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

特点

dNTPs included
hotstart

技术

PCR: suitable

颜色

colorless

应用

agriculture

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。

应用

Extract-N-Amp™植物PCR试剂盒用于从植物和真菌菌丝体中分离基因组DNA，还用于聚合酶链式反应（PCR）。
Extract-N-Amp™植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。

特点和优势

  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化，节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

组分

Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。

其他说明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。
Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Shane Wright et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(20), 7718-7722 (2006-05-05)
Using an appropriately designed and replicated study of a latitudinal influence on rates of evolution, we test the prediction by K. Rohde [(1992) Oikos 65, 514-527] that the tempo of molecular evolution in the tropics is greater than at higher
Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
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Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
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The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita
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商品

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

实验方案

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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