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Merck
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XNAP2

Sigma-Aldrich

Extract-N-Amp植物PCR试剂盒

sufficient for 100 extractions, sufficient for 100 amplifications

别名:

Plant direct PCR kit

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

用途

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

特点

dNTPs included
hotstart

技术

PCR: suitable

颜色

colorless

应用

agriculture

运输

wet ice

储存温度

−20°C

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一般描述

Extract-N-Amp植物PCR试剂盒用于直接PCR(聚合酶链反应)。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案,无需常规的植物组织冷冻处理。

应用

Extract-N-Amp植物PCR试剂盒用于从植物和真菌菌丝体中分离基因组DNA,还用于聚合酶链式反应(PCR)。
Extract-N-Amp植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。

特点和优势

  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化,节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

组分

Extract-N-Amp植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物(2X)(缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体)。

其他说明

更多信息,请见www.sigma-aldrich.com/extract-n-amp
Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Shane Wright et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(20), 7718-7722 (2006-05-05)
Using an appropriately designed and replicated study of a latitudinal influence on rates of evolution, we test the prediction by K. Rohde [(1992) Oikos 65, 514-527] that the tempo of molecular evolution in the tropics is greater than at higher
Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
José Dijair Antonino de Souza Júnior et al.
PloS one, 8(12), e85364-e85364 (2014-01-07)
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita

商品

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA,包括哺乳动物、植物和细菌培养物来源的细胞和组织。

实验方案

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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