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一般描述
Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。
应用
Extract-N-Amp™植物PCR试剂盒适用于基因分型。
它已被用于从新鲜蘑菇中提取DNA。
它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp™组织PCR试剂盒用于从新鲜蘑菇中提取DNA。
特点和优势
- PCR用植物基因组DNA可以在15分钟内提取。
- 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
- 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
- 热启动抗体确保基因组DNA的高度特异性PCR扩增
- 适用于高通量植物基因分析
- 无需长时间的酶消化，节省时间和精力
- 提取的DNA在4 °C 下至少6个月保持稳定。
组分
Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。
法律信息
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
价格
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
Rapid and reliable high-throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms
Dentinger BTM, et al.
Molecular Ecology Resources, 10(4), 628-633 (2010)
Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Jian-Feng Li et al.
Plant methods, 6(1), 1-1 (2010-02-26)
Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed
商品
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
实验方案
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
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