packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
orange to green
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC FOXO1/PAX7 Dual Color Single Fusion Probe (PL17) is a direct-labeled FISH probe designed for the qualitative detection of translocation t(1;13)(p36.1;q14.1) involving the human FOXO1 and PAX7 genes in formalin-fixed, paraffin-embedded specimens, by fluorescence in situ hybridization (FISH). The probe is intended for use with the ZytoLight FISH-Tissue Implementation Kit.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FOXO1/PAX7 Dual Color Single Fusion Probe comprises:
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FOXO1/PAX7 Dual Color Single Fusion Probe comprises:
- ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~4.5 ng/µl), targeting sequences mapping in 13q14.11* (chr13:40,816,168-41,132,595) proximal to the FOXO1 breakpoint region;
- ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10 ng/µl), targeting sequences mapping in 1p36.13* (chr1:18,139,970-18,956,785) distal to the PAX7 breakpoint region;
- Formamide based hybridization buffer
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
Application
This probe is intended for use in formalin-fixed, paraffin-embedded specimens to qualitatively detect translocation t(1;13)(p36.1;q14.1) involving the human FOXO1 and PAX7 genes, via FISH.
Analysis Note
Using suitable filter sets, the hybridization signals of the probe appear orange near the FOXO1 breakpoint region and green away from the PAX7 breakpoint region.
- Normal scenario: in of normal cells during interphase or cells without a translocation of the respective gene regions, two separate green and orange signals are observed.
- Aberrant scenario: gene fusion is indicated by one separate orange signal, one separate green signal, and one orange/green fusion signal.
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
警示词
Danger
危险代码
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
靶器官
Blood
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects