packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
green to orange
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC FGFR1/CEN 8 Dual Color Probe (PL29) is a direct-labeled FISH probe for the qualitative detection of amplifications of the human FGFR1 gene and the detection of chromosome 8 alpha satellites in formalin-fixed, paraffin-embedded specimens, using fluorescence in situ hybridization (FISH). The probe is intended for use with the ZytoLight FISH-Tissue Implementation Kit.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FGFR1/CEN 8 Dual Color Probe comprises:
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FGFR1/CEN 8 Dual Color Probe comprises:
- ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10.0 ng/µl), targeting sequences mapping in 8p11.22-p11.23* (chr8:38,063,906-38,527,745) harboring the FGFR1 gene region;
- ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~1.5 ng/µl), targeting sequences mapping in 8p11.1-q11.1 specific for the alpha satellite centromeric region D8Z2 of chromosome 8;
- Formamide based hybridization buffer
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
Application
This probe is intended for use in formalin-fixed, paraffin-embedded specimens, to qualitatively detect amplifications of the human FGFR1 gene and the detection of chromosome 8 alpha satellites, via FISH.
Analysis Note
Using suitable filter sets, the hybridization signals of the probe appear green at the FGFR1 gene region and orange at CEN 8.
- Normal scenario: in normal cells during interphase or cells without an amplification of the FGFR1 gene region, two green signals and two orange signals are observed.
- Aberrant scenario: in cells with an amplification of the FGFR1 gene region, more green signals or green signal clusters are observed.
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
警示词
Danger
危险代码
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
靶器官
Blood
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects