ZytoLight^® SPEC 19q13/19p13 Dual Color Probe

The ZytoLight^® SPEC 19q13/19p13 Dual Color Probe (PL35) is a direct-labeled FISH probe for the qualitative detection of deletions of the human chromosomal region 19q13.32-q13.33 and the detection of chromosome 19p13.3 specific sequences in formalin-fixed, paraffin-embedded specimens, using fluorescence in situ hybridization (FISH). The probe is intended for use with the ZytoLight FISH-Tissue Implementation Kit.

ZytoLight^® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.

Components

The ZytoLight SPEC 19q13/19p13 Dual Color Probe comprises:

• ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~1.5 ng/µl), targeting sequences mapping in 19q13.32-q13.33* (chr19:47,857,776-48,374,564);
• ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10.0 ng/µl), targeting sequences mapping in 19p13.3* (chr19:658,555-1,144,465);
• Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19

Principle

The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.

This probe is intended for use in formalin-fixed, paraffin-embedded specimens to qualitatively detect deletions of the human chromosomal region 19q13.32-q13.33 and the detection of chromosome 19p13.3 specific sequences, via FISH.

Using suitable filter sets, the hybridization signals of the probe appear orange at the 19q13 locus and green at the 19p13 locus.

• Normal scenario: in normal cells during interphase or cells without a deletion of the 19q13 locus, two orange signals and two green signals are observed.
• Aberrant scenario: in cells with deletion of the 19q13 locus, fewer orange signals are observed. Partial deletions might show a normal signal pattern with smaller orange signals.

Overlapping signals may appear yellow.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany