ZytoLight^® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe

The ZytoLight^® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe (PL40, Z-2081-50/200) is a FISH probe designed for the qualitative detection of the human CDKN2A gene and the alpha-satellite regions of chromosomes 3, 7, and 17 in formalin-fixed, paraffin-embedded specimens using fluorescence in situ hybridization (FISH). This probe employs ZyGold-labeled polynucleotides for the CDKN2A gene at 9p21.3 (chr9:21,742,629-22,056,853, ~5.5 ng/µl), ZyRed for chromosome 3 (D3Z1, 3p11.1-q11.1, ~0.5 ng/µl), ZyGreen for chromosome 7 (D7Z1, 7p11.1-q11.1, ~4.5 ng/µl), and ZyBlue for chromosome 17 (D17Z1, 17p11.1-q11.1, ~12 ng/µl). It is intended for use with the ZytoLight FISH-Tissue Implementation Kit (Prod. No. Z-2028-5/-20).

Principle:

The ZytoLight^® system uses fluorescence in situ hybridization (FISH) to detect and visualize specific nucleic acid sequences in FFPE specimens. Direct-labeled probes co-denature with target DNA strands for hybridization. Unbound probes are washed away, and hybridized fragments are counterstained with DAPI.The ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe includes:-ZyGold: Targets the CDKN2A gene at 9p21.3 (excitation 532 nm/emission 553 nm).-ZyRed: Targets chromosome 3 (excitation 580 nm/emission 599 nm).-ZyGreen: Targets chromosome 7 (excitation 503 nm/emission 528 nm).-ZyBlue: Targets chromosome 17 (excitation 418 nm/emission 467 nm).FISH visualizes gold, red, green, and blue signals corresponding to these targets. The microscopy setup eliminates the need for antibodies, using exciter, dichroic, and barrier filters for precise excitation and emission, optimized for detecting genetic aberrations like deletions and aneuploidies in tissue sections.

Components:

Probe Composition:

• ZyGold: Labeled polynucleotides (~5.5 ng/µl) for the CDKN2A gene region.
• ZyRed: Labeled polynucleotides (~0.5 ng/µl) for chromosome 3.-ZyGreen: Labeled polynucleotides (~4.5 ng/µl) for chromosome 7.
• ZyBlue: Labeled polynucleotides (~12 ng/µl) for chromosome 17.
• Hybridization Buffer: Formamide-based hybridization buffer.

Product Sizes:-Z-2081-50: 0.05 ml (5 reactions of 10 µl each).-Z-2081-200: 0.2 ml (20 reactions of 10 µl each).Related Products: Intended for use with the ZytoLight FISH-Tissue Implementation Kit (Prod. No. Z-2028-5/-20).

ZytoLight® SPEC CDKN2A/CEN 3/7/17 Quadruple Color Probe aids differential diagnosis of various cancers by detecting CDKN2A deletions/aneusomies of chromosomes 3/7/17 in FFPE tissues

For the qualitative detection of the human CDKN2A gene as well as alpha-satellites of chromosomes 3, 7, and 17 by fluorescence in situ hybridization (FISH)

In normal interphase nuclei, the expected results are two gold (CDKN2A), two red (CEN 3), two green (CEN 7), and two blue (CEN 17) signals. In cases of deletion of the CDKN2A gene locus, a reduced number of gold signals will be observed. Cells with aneuploidy of chromosomes 3, 7, or 17 may show varying numbers of signals corresponding to those chromosomes.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany