packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
green to orange
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC BCR/ABL1 Dual Color Dual Fusion Probe is a direct-labeled fluorescence in situ hybridization (FISH) probe designed for the qualitative detection of the t(9;22)(q34.1;q11.2) chromosomal translocation involving the BCR and ABL1 genes, commonly associated with chronic myeloid leukemia (CML). For optimal performance, the probe is intended for use with the ZytoLight® FISH-Cytology Implementation Kit.
ZytoLight® probes enable the precise identification of genetic abnormalities—including translocations, deletions, amplifications, and chromosomal aneuploidies—in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC BCR/ABL1 Dual Color Dual Fusion Probe comprises:
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
ZytoLight® probes enable the precise identification of genetic abnormalities—including translocations, deletions, amplifications, and chromosomal aneuploidies—in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC BCR/ABL1 Dual Color Dual Fusion Probe comprises:
- ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~12 ng/µl), targeting sequences mapping in 22q11.22-q11.23* (chr22:23,000,029-24,431,064) harboring the BCR gene region;
- ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~6 ng/µl), targeting sequences mapping in 9q34.11-q34.13* (chr9:133,223,081-134,103,849) harboring the ABL1 gene region;
- Formamide based hybridization buffer
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
Application
For the qualitative detection of translocation t(9;22)(q34.1;q11.2) by fluorescence in situ hybridization (FISH)
Analysis Note
Using suitable filter sets, the probe’s hybridization signals display as green for the BCR gene region and orange for the ABL1 gene region.
Normal scenario: normal cells or in cells lacking a translocation involving these gene regions, two distinct signals are observed: one green and one orange during interphase.
Aberrant scenario: where gene fusion has occurred, the signal pattern consists of one green signal, one orange signal, and two combined orange/green fusion signals.
Overlapping orange and green signals may appear yellow.
Normal scenario: normal cells or in cells lacking a translocation involving these gene regions, two distinct signals are observed: one green and one orange during interphase.
Aberrant scenario: where gene fusion has occurred, the signal pattern consists of one green signal, one orange signal, and two combined orange/green fusion signals.
Overlapping orange and green signals may appear yellow.
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
警示词
Danger
危险代码
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
靶器官
Blood
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
闪点 (F)
Not applicable
闪点 (°C)
Not applicable