ZytoLight^® SPEC ALK/2q11 Dual Color Probe

The ZytoLight SPEC ALK/2q11 Dual Color Probe (PL117) is a direct-labeled FISH probe designed for the qualitative detection of amplifications involving the human ALK gene and the detection of chromosome 2q11 specific sequences in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used with the ZytoLight FISH-Tissue Implementation Kit.

Components:

ZytoLight® SPEC ALK/2q11 Dual Color Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting the ALK gene region on chromosome 2 (2p23.1).
• ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting sequences specific to chromosome 2q11.
• Formamide-based hybridization buffer.

Principle:

Fluorescence in situ hybridization (FISH) uses direct-labeled probes (ALK, 2q11) that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.

The ZytoLight^® SPEC ALK/2q11 Dual Color Probe is used to detect RET gene rearrangements and ALK copy number gains/amplifications in formalin-fixed, paraffin-embedded tissue specimens, to support molecular characterization within the analytical workflow. In addition to RET rearrangement detection, ALK copy number alterations may be observed in a variety of tissue types, providing information about gene copy status within the context of the assay.ance.

In a normal interphase nucleus, two green signals (for ALK) and two orange signals (for the 2q11 locus) are expected. In cases of amplification, an increased number of green signals or clusters will be observed. Any unexpected signal patterns should be further investigated for potential genetic rearrangements.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany