ZytoLight^® SPEC ROS1/CEN 6 Dual Color Probe

The ZytoLight SPEC ROS1/CEN 6 Dual Color Probe (PL118) is a direct-labeled FISH probe intended for the qualitative detection of amplifications involving the human ROS1 gene and for detecting chromosome 6 alpha satellites in formalin-fixed, paraffin-embedded (FFPE) specimens. It is designed for use in conjunction with the ZytoLight FISH-Tissue Implementation Kit.

Components:

ZytoLight® SPEC ROS1/CEN 6 Dual Color Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting sequences in the ROS1 gene region on chromosome 6 (6q22.1).
• ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting the alpha satellite centromeric region D6Z1 of chromosome 6.
• Formamide-based hybridization buffer.

Principle:

Fluorescence in situ hybridization (FISH) uses direct-labeled probes (ROS1, CEN 6) that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.

ZytoLight^® SPEC ROS1/CEN 6 Dual Color Probe is used to detect ROS1 gene amplifications and alpha satellite regions on chromosome 6, aiding in differential analyses where ROS1 alterations are relevant. The probe is designed to be used in conjunction with the ZytoLight FISH-Tissue Implementation Kit (Z-2028-5/-20).

In a normal interphase nucleus, two green signals (for ROS1) and two orange signals (for chromosome 6) are expected. In cases of amplification or translocation, an increased number of green or orange signals may be observed, which should be further investigated for potential genetic rearrangements.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany