ZytoLight^® SPEC YWHAE Dual Color Break Apart Probe

The ZytoLight SPEC YWHAE Dual Color Break Apart Probe (PL134) is a direct-labeled FISH probe designed for the qualitative detection of translocations involving the human YWHAE gene at 17p13.3 in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used in combination with the ZytoLight FISH-Tissue Implementation Kit.

Components:

ZytoLight® SPEC YWHAE Dual Color Break Apart Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting sequences proximal to the YWHAE breakpoint region on -chromosome 17 (17p13.3).
• ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting sequences distal to the YWHAE breakpoint region on chromosome 17.
• Formamide-based hybridization buffer.

Principle:
FISH uses direct-labeled probes flanking YWHAE breakpoint that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy per ZytoLight FISH-Tissue Implementation Kit (Z-2028-5/-20).

ZytoLight® SPEC YWHAE Dual Color Break Apart Probe aids in the differential analysis of conditions associated with YWHAE rearrangements.. It is used for the qualitative detection of translocations involving the human YWHAE gene at 17p13.3 by fluorescence in situ hybridization (FISH).

In a normal interphase nucleus, two green and two orange signals are expected. In cases of translocation, the presence of separate green and orange signals will indicate an alteration. Any unexpected signal patterns should warrant further investigation to confirm genetic rearrangements.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany