Cancer Cell Culture: Methods and Protocols

This volume describes new techniques for the culture of cancer cells. The first section introduces the rationale behind selection of specific materials to help the researcher select culture conditions appropriate to their studies and discusses general techniques operating in many culture facilities. The second section covers specific requirements of individual cancer cell types for optimal growth and maintenance. A range of procedures are described in section three which include assays to evaluate proliferation, viability, cytotoxicity, apoptosis, migration, invasion, and angiogenesis. Techniques of gene transfer and the development of drug resistance are also described. The book concludes with methods of co-culture of different cell types.